Anti-RNA hairpin mAbs

Cat. No.

MABL-2839

Name

Anti-RNA hairpin mAbs

Clone No.

AFD- BL3-6S97N

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization

Species Reactivity

Species independent

Basic Information

Specificity

The antibody binds to the RNA hairpins GAAACAC   hairpin. An RNA hairpin is an essential secondary structure of RNA. It can   guide RNA folding, determine interactions in a ribozyme, protect messenger   RNA (mRNA) from degradation, serve as a recognition motif for RNA binding   proteins or act as a substrate for enzymatic reactions.

Alternative Name

UniProt

Immunogen

A library of Fabs was generated by   rationally varying the composition of the residues in complementarity   determining regions (CDRs) of Fab BL3–6. Subsequently, the Fab library was   challenged in a phage display selection to recognize and bind the GAAACAC   motif.

Application Notes

The Fab was generated by directed mutagenesis from the parental Fab BL3–6. The S97N mutation in the   complementarity determining region L3 of the Fab improved the binding   affinity compared to the parent Fab BL3–6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity- matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3–6S97N was determined (Koirala et al., 2018; PMID: 29309709).

Antibody First   Published

Koirala et al. Affinity maturation of a   portable Fab–RNA module for chaperone-assisted RNA crystallography Nucleic   Acids Res. 2018 Mar 16;46(5):2624-2635. PMID:29309709

Note on publication

The original paper describes the use of Fab   fragment as chaperone for RNA crystallography.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.