Key features and details | |
Cat. No. | MABL-2839 |
Name | Anti-RNA hairpin mAbs |
Clone No. | AFD- BL3-6S97N |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody binds to the RNA hairpins GAAACAC hairpin. An RNA hairpin is an essential secondary structure of RNA. It can guide RNA folding, determine interactions in a ribozyme, protect messenger RNA (mRNA) from degradation, serve as a recognition motif for RNA binding proteins or act as a substrate for enzymatic reactions. |
Alternative Name | |
UniProt | |
Immunogen | A library of Fabs was generated by rationally varying the composition of the residues in complementarity determining regions (CDRs) of Fab BL3–6. Subsequently, the Fab library was challenged in a phage display selection to recognize and bind the GAAACAC motif. |
Application Notes | The Fab was generated by directed mutagenesis from the parental Fab BL3–6. The S97N mutation in the complementarity determining region L3 of the Fab improved the binding affinity compared to the parent Fab BL3–6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity- matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3–6S97N was determined (Koirala et al., 2018; PMID: 29309709). |
Antibody First Published | Koirala et al. Affinity maturation of a portable Fab–RNA module for chaperone-assisted RNA crystallography Nucleic Acids Res. 2018 Mar 16;46(5):2624-2635. PMID:29309709 |
Note on publication | The original paper describes the use of Fab fragment as chaperone for RNA crystallography. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |