欢迎来到西安迈博睿(Mabioway)生物科技有限公司 Call: 15339069646 | E-mail: info@mabioway.cn 在线留言 |

服务热线

15339069646
网站导航
当前位置: 首页 > 产品展示 > 抗体产品 > 一抗 > 其他抗体
其他抗体

Anti-RNA hairpin mAbs

简要描述:

The antibody binds to the RNA hairpins GAAACAC hairpin. An RNA hairpin is an essential secondary structure of RNA. It can guide RNA folding, determine interactions in a ribozyme, protect messenger RNA (mRNA) from degradation, serve as a recognition motif for RNA binding proteins or act as a substrate for enzymatic reactions.

产品参数:

Cat. No.:MABL-2839

Species:Engineer

Species Reactivity:Species independent

Type:Recombinate

Application:crystallization

详细介绍

Key features and   details


Cat. No.

MABL-2839

Name

Anti-RNA hairpin mAbs

Clone No.

AFD- BL3-6S97N

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization

Species Reactivity

Species independent

Basic Information


Specificity

The antibody binds to the RNA hairpins GAAACAC   hairpin. An RNA hairpin is an essential secondary structure of RNA. It can   guide RNA folding, determine interactions in a ribozyme, protect messenger   RNA (mRNA) from degradation, serve as a recognition motif for RNA binding   proteins or act as a substrate for enzymatic reactions.

Alternative Name


UniProt


Immunogen

A library of Fabs was generated by   rationally varying the composition of the residues in complementarity   determining regions (CDRs) of Fab BL3–6. Subsequently, the Fab library was   challenged in a phage display selection to recognize and bind the GAAACAC   motif.

Application Notes

The Fab was generated by directed mutagenesis from the parental Fab BL3–6. The S97N mutation in the   complementarity determining region L3 of the Fab improved the binding   affinity compared to the parent Fab BL3–6 (Kd = 5 nM). The Fab fragment was also found to bind the affinity- matured GAGACCC hairpin. The crystal structure of the Spinach RNA grafted with GAAACAC loop in complex with Fab BL3–6S97N was determined (Koirala et al., 2018; PMID: 29309709).

Antibody First   Published

Koirala et al. Affinity maturation of a   portable Fab–RNA module for chaperone-assisted RNA crystallography Nucleic   Acids Res. 2018 Mar 16;46(5):2624-2635. PMID:29309709

Note on publication

The original paper describes the use of Fab   fragment as chaperone for RNA crystallography.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


服务咨询

留言框

  • 标题:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

推荐产品

联系我们

Copyright © 2012-2029 西安迈博睿(Mabioway)生物科技有限公司 版权所有

地址:陕西省西安市浐灞生态区广运潭大道南段4555号长安大学科技园4号楼A单元2层

电话:15339069646 邮箱:info@mabioway.cn

陕ICP备2021006339号