Anti-Rituximab (variable region) mAbs

Cat. No.

MABL-2838

Name

Anti-Rituximab (variable region) mAbs

Clone No.

AFD- MB2A4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, functional assay, IP, SPR, WB,   ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is an idiotypic antibody   against rituximab. It shows no cross-reactivity with other CD20-specific   antibodies. Rituximab is a monoclonal antibody against CD20 used to treat   autoimmune diseases and some types of cancer.

Alternative Name

Rituximab; Rituxan

UniProt

Immunogen

The rat version of this antibody was raised   by immunizing a rat with the F(ab)2 fragment of Rituximab.

Application Notes

To develop an ELISA to measure rituximab, an   ELISA was performed on solutions of rituximab using the rat version of this antibody. The ELISA could be used to quantify rituximab at concentrations between 0.125 and 50 μg/mL. Furthermore, the binding strength of this antibody to rituximab was measured using SPR. It showed a Kd value of 0.6 nM.   Finally, a competition assay between this antibody and a P20 peptide was   performed to determine whether P20 could block the binding of this antibody. A decrease in binding was indeed noted (Blasco et al., 2007; PMID: 17651747).   To characterize this antibody, an ELISA was performed on diluted samples of   rituximab using the rat version of this antibody. Furthermore, flow cytometry   was used to detect rituximab on the surface of CD20-positive cells using this   antibody. The antibody was bound to rituximab-coated cells and showed no   cross-reactivity with other CD20-binding antibodies. The ability of this   antibody to block rituximab from binding B cells was also shown (Cragg et al.,   2004; PMID: 15213098).

Antibody First   Published

Cragg et al. A new anti-idiotype antibody   capable of binding rituximab on the surface of lymphoma cells Blood. 2004 Oct 15;104(8):2540-2. PMID:15213098

Note on publication

An anti-idiotype antibody against rituximab   was raised and characterized.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.