Key features and details | |
Cat. No. | MABL-2800 |
Name | Anti-RANK-L mAbs |
Clone No. | AFD- 3K7F5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human-RANK-L. RANKL is a tumor necrosis factor related protein that binds to, and activates, the TNF receptor related protein Receptor Activator of NF-kB (RANK). RANKL acts as the key cytokine that regulates osteoclast differentiation and activation during normal bone remodeling and during disease |
Alternative Name | CD254; TRANCE; RANKL; PAE30; ODF; OPGL; Tumor necrosis factor ligand superfamily member 11; Osteoclast differentiation factor; Osteoprotegerin ligand; Receptor activator of nuclear factor kappa-B ligand; TNF-related activation-induced cytokine |
UniProt | O14788 |
Immunogen | The original antibody was generated by panning a phage display library against human sRANKL. |
Application Notes | The affinity of the original format of the antibody is 0.17x 10-12 nM, as measured by PAE technology. RAW264.7, a murine macrophage cell line that can be induced by RANKL to differentiate into osteoclast-like cells, was used in a TRAP test. In the TRAP test, the original antibody or the Fab fragment were able to neutralize the stimulating effect of RANKL on osteoclast formation from a RAW 264.7 culture. Further, the addition of the original format of the antibody or the Fab fragment inhibited osteoclast differentiation of the RAW cell in a dose-dependent manner. The affinity of the original format and the Fab fragment of the antibody was measured by the Scatchard method (affinity = 8.7 and 6.5 pM). The original antibody and the Fab fragment could significantly increase the bone densities and bone calcium content in a dose-dependent manner, in in vivo experiments (WO2011116527A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
