Key features and details | |
Cat. No. | MABL-2535 |
Name | Anti-Occludin mAbs |
Clone No. | AFD- 37-5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ICC, immunoblot, in vitro, in vivo, inhibition, IP, TER, ELISA, FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for the first extracellular loop (EL1) of OCLN. |
Alternative Name | OCLN |
UniProt | Q16625 |
Immunogen | The original antibody was generated by immunizing Wistar rats with a mammalian expression vector encoding hOCLN. |
Application Notes | The cell binding capability of the original format of this antibody (rat IgG2b) was assessed using FC, and its binding to HT1080/hOCLN cells was assessed using a cell-based ELISA. This antibody was found to have no negative effect on tight junction (TJ) barrier function, as determined by a TJ barrier function assay (TER). Furthermore, this antibody was found to have no negative effect on cell viability, as determined by a colorimetric XTT assay. This antibody was found to strongly inhibit (in vitro) and fully block (in vivo) HCV infections, as measured by ICC (Shimizu et al., 2018; PMID: 29437969). This antibody's Kd for hOCLN is 0.720 ± 0.084 nM, as measured in a cell-based competition ELISA. This antibody successfully precipitated FLAG-hOCLN proteins found in HT1080 cell lysates and endogenously expressed cellular OCLN found in Caco-2, HepG2, and MCF-7 cell lysates. |
Antibody First Published | Shimizu et al. Monoclonal Antibodies against Occludin Completely Prevented Hepatitis C Virus Infection in a Mouse Model J Virol. 2018 Mar 28;92(8):e02258-17. doi: 10.1128/JVI.02258-17. Print 2018 Apr 15. PMID:29437969 |
Note on publication | The original publication discusses the successful generation of four rat monoclonal antibodies that recognize the extracellular domains of the tight junction protein occludin and the potential use of these antibodies as promising candidates for novel anti-HCV agents. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
