Key features and details | |
Cat. No. | MABL-2575 |
Name | Anti-p120/catenin mAbs |
Clone No. | AFD- 2B12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB |
Species Reactivity | Chicken, Hamster, Rat, Human, Mouse |
Basic Information | |
Specificity | The antibody recognizes mouse, rat, hamster, human and chicken p120/catenin, but it does not cross react with the porcine and canine homologs. The epitope is located at amino acid residues 55‐101. |
Alternative Name | Catenin delta-1; Cadherin-associated Src substrate; CAS; p120 catenin; p120(ctn); p120(cas) |
UniProt | O60716 |
Immunogen | The original antibody was generated immunizing A/J mice with tyrosine phosphoproteins purified from chicken embryonic fibroblasts (CEFs) expressing pp60/c-src 527F and 2A/527F. |
Application Notes | The antibody (IgG1) was used to immunoprecipitate tyrosine phosphoprotein P120. The antibody was used in Western blot (Kanner at al, 1990; PMID:2110361). The antibody was used to analyse the level of tyrosine phosphorylation of P120 in uninfected CE cells and cells expressing several oncogenic and nononcogenic src variants. The antibody was used to immunoprecipitate P120 from CE cell extracts, then the antibody was used in western blot (Kanner et al, 1991; PMID:1703631). The antibody was employed to perform immunocytochemical analysis in order to investigate the cellular localization of p120 on CMT 93/69 and MDCK cells (Staddon et al, 1995; PMID: 7615637). The antibody immunoprecipitated only CAS1 isoforms (Mo et al., 1996). The antibody was used for immunoprecipitation and Western blotting of pp120 from MDCK cell lysates (Dillon et al, 1998; PMID: 9422525). |
Antibody First Published | Kanner et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc Natl Acad Sci U S A.1990 May;87(9):3328-32. PMID:2110361 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
