Anti-p-tau mAbs

Cat. No.

MABL-2573

Name

Anti-p-tau mAbs

Clone No.

AFD- pS396/pS404_1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for phosporilated   human tau protein.

Alternative Name

ptau; phospho-tau

UniProt

Immunogen

The original antibody was generated by   immunizing chickens with phosphorylated tau peptides conjugated to KLH,   constructing an scFv phagemid library from spleen and bone marrow RNA, and   selecting specific binders through ELISA screening of the phage library   against biotinylated phospho- peptides.

Application Notes

The specificity of the original antibody   (chicken scFv, lambda) was evaluated via an ELISA. A chimeric human IgG1   version of the antibody was generated. The chimeric variant preserved the   original binding characteristics as demonstrated in an ELISA. The binding   affinity (KD) of the chimeric antibody was measured at 2.045 µM via SPR (Shih   et al., 2012; PMID: 23148212).

Antibody First   Published

Shih et al. An ultra-specific avian antibody   to phosphorylated tau protein reveals a unique mechanism for phosphoepitope recognition. J Biol Chem. 2012 Dec 28;287(53):44425-34. doi:   10.1074/jbc.M112.415935. Epub 2012 Nov 12. PMID:23148212

Note on publication

The original publication describes the   generation and structural characterization of highly specific recombinant   chicken antibodies against three phosphorylated tau epitopes associated with   Alzheimer's disease.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.