Key features and details | |
Cat. No. | MABL-2462 |
Name | Anti-NCAM-L1 mAbs |
Clone No. | AFD- H2 scFv |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Activate, WB, ELISA, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This scFv binds specifically to the fibronectin type III repeats 1–2 of mouse L1 with a KD of around 2μM (determined by ELISA). It does not cross-react with human L1 in ELISAs, Western blots or immunofluorescence staining of cultured neurons. L1 plays important roles in cell adhesion, neuronal migration, neurite outgrowth, fasciculation and pathfinding, neuronal survival, and synaptic plasticity. L1 is not recongised under reducing conditions. L1 is a membrane glycoprotein of the immunoglobulin superfamily. Its interacts with adhesion molecules and proteins of the ECM. L1 also is inolved in the migration of cerebellar granule cells, enhancing neuronal survival and memory. |
Alternative Name | CD171; L1; Neural cell adhesion molecule L1; N-CAM-L1; L1 |
UniProt | P11627 |
Immunogen | single-chain variable fragment antibodies against mouse L1 from a human synthetic phage display library. |
Application Notes | This scFv can stimulate neurite outgrowth from cerebellar dorsal, rootganglion and motor neurons and also be used to enhance survival of cerevellar neurons by facilitating the clustering of L1. H2 can also be used for ELISA, WB and IF studies. |
Antibody First Published | Dong et al. Single chain Fv antibodies against neural cell adhesion molecule L1 trigger L1 functions in cultured neurons. Mol Cell Neurosci. 2003 Feb;22(2):234-47. PMID:12676533 |
Note on publication | Describes the generation and function of scFvs against mouse L1 protein. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
