Anti-NC174 mAbs

Cat. No.

MABL-2461

Name

Anti-NC174 mAbs

Clone No.

AFD- NC10.14

From

Recombinant Antibody

Isotype

Engineer antibody

Application

CD, FPA, RIA, IF

Species Reactivity

Species independent

Basic Information

Specificity

This antibody specifically binds the hapten   NC174, a trisubstituted guanidinium sweetener.

Alternative Name

N-(p-cyanophenyl)-N'-(diphenylmethyl)-N''-(carboxymethyl)guanidine;   N- (P-CYANOPHENYL)-N'-DIPHENYLMETHYL-GUANIDINE-ACETIC ACID;   N-(pcyanophenyl)-N'-(diphenylmethyl)guanidine acetic acid; Trisubstituted   guanidine sweetener; TGS

UniProt

Immunogen

The original antibody was generated by   murine hybridomas produced by traditional techniques; mice were immunized   with TGS covalently conjugated to bovine serum albumin carrier protein   through one of the phenyl rings in Freund's complete adjuvant.

Application Notes

Ligand-induced quenching of IgG NC10.14 was   used to determine the dissociation constant of the NC10.14-NC174 complex via   IF and Circular Dichroism (CD) Spectroscopy (Viswanathan et al., 1996; PMID:   8756519). This antibody was used to study the ligand specificity of the   putative sweet taste receptor binding site. Furthermore, the Fab of this   antibody was used to show that aromatic-aromatic contacts at the interdomain   surfaces are essential in the origination of CD spectra for both the near-   and far- UV region (Tetin et al., 1996; PMID: 8573581). This antibody was   examined to determine molecular recognition specificities using 14 different   sweetener analogs in a competitive radioimmunoassay (Anchin et al., 1997;   PMID: 9587873).

Antibody First   Published

Anchin et al. Variable region sequence and characterization of monoclonal antibodies to a N,N',N"-trisubstituted   guanidine high potency sweetener Mol Immunol. 1993 Nov; 30(16):1463-71. doi: 10.1016/0161-5890(93)90108-n PMID:8232332

Note on publication

The original publication explores the   creation and characterization of a monoclonal antibodies library that targets   a trisubstituted guanidinium sweetener.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.