Key features and details | |
Cat. No. | MABL-2339 |
Name | Anti-Melanin mAbs |
Clone No. | AFD- 6D2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunotherapy, radioimmunotherapy, ELISA, IF, IHC |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds melanin. Melanin is a natural pigment found in most organisms is produced in a specialized group of cells called the melanocytes. |
Alternative Name | CHEBI:89634 |
UniProt | |
Immunogen | The original antibody was generated by immunizing 5-8 weeks old female BALB/c mice intraperitoneally with L-dopa melanin ghosts suspended in a 1:1 (vol/vol) emulsion of complete Freund's adjuvant. Digestion of in vitro-melanized C. neoformans cells with proteases, denaturant, and hot concentrated acid yields melanin particles that retain the shape of fungal cells and are therefore called melanin ghosts. |
Application Notes | Cryptococcus neoformans is an encapsulated fungus that causes life-threatening meningoencephalitis, predominantly in immunocompromised individuals. C. neoformans has a laccase (CNLAC1) that catalyzes the synthesis of polymerized melanin in vitro when the cells are grown in the presence of phenolic substrates, such as 3,4-dihydroxyphenylalanine (L-dopa). The ability of Cryptococcus neoformans to synthesize polymerized melanin in vitro has been associated with virulence. The binding characterization of this antibody towards L-dopa melanin, synthetic melanin and melanin from Sepia officinalis was carried out using ELISA. The original mouse antibody was also used for the immunofluorescence stainings of lung and brain tissues from Mel+ or Mel− C. neoformans-infected rodents (PMID: 10768981). |
Antibody First Published | Rosas et al. Synthesis of polymerized melanin by Cryptococcus neoformans in infected rodents. Infect Immun. 2000 May; 68(5): 2845–2853. PMID:10768981 |
Note on publication | Antibodies against Cryptococcus neoformans melanin were generated to be used in immunohistochemical studies of C. neoformans-infected rodents and to identify whether the fungus synthesizes polymerized melanin during the infection. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
