Key features and details | |
Cat. No. | MABL-2338 |
Name | Anti-Melan-A mAbs |
Clone No. | AFD- A103 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC, IP, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human Melan-A protein. This protein is found on the surface of melanocytes and is an important marker for detection of melanocytic tumors. |
Alternative Name | MART-1; MART1; Melan A; MLANA; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A; Melanoma antigen recognized by T-cells 1; MALENA |
UniProt | Q16655 |
Immunogen | The original antibody was generated by subcutaneously immunizing BALB/c mice with recombinant Melan-A protein in complete Freund's adjuvant. |
Application Notes | The binding characterization of this antibody towards Melan-A was done using ELISA. The original mouse IgG1 antibody was able to detect Melan-A from cell lysates of SK-MEL-13, SK-MEL-23, and SK-MEL-28 cell lines in an immunoblot assay. This antibody was also able to immunoprecipitate Melan-A protein from cell lysates of SK-MEL-13, SK-MEL-23, and SK-MEL-28 cell lines. The original mouse antibody was also used in the immunohistochemical analysis of normal skin and two Melan-A positive melanomas SK- MEL-19 and MZ2-MEL3.1 (PMID: 8650193). This antibody was used for detection of Melan-A antigen on formalin-fixed, paraffin-embedded tissues and in diagnosis of metastatic melanoma (PMID: 9591730). Immunohistochemical analysis with this antibody was useful in the differential diagnosis of adrenocortical neoplasms from both renal cell and hepatocellular carcinomas (PMID: 14663359; 9422316). |
Antibody First Published | Chen et al. Serological analysis of Melan-A(MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5915-9. PMID:8650193 |
Note on publication | Describes the generation of this antibody and mentions its use in the characterization of the antigen (Melan-A) that it binds. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
