Key features and details | |
Cat. No. | MABL-1561 |
Name | Anti-FAP mAbs |
Clone No. | AFD- 3F2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC, IHC |
Species Reactivity | Human, Cynomolgus Monkey, Mouse |
Basic Information | |
Specificity | This antibody can bind human, mouse and cynomolgus monkey fibroblast activation protein. |
Alternative Name | Seprase; Fibroblast activation protein; Prolyl endopeptidase FAP; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; FAPalpha; Fibroblast activation protein alpha; Gelatin degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Serine integral membrane protease; SIMP; Surface-expressed protease |
UniProt | Q12884 |
Immunogen | |
Application Notes | The original mouse antibody was used to check the expression levels of FAP on the surface of GM05389 lung fibroblasts using flow cytometry. The original mouse IgG1 version of this antibody binds human, mouse and cynomolgus monkey FAP with an avidity of 39 pM, 29 pM and 42 pM respectively. Binding of human IgG1 antibody 3F2 to human and murine FAP expressed on stably transfected HEK293 cells, human fibroblasts cell line GM05389 was measured by floy cytometry. The mouse IgG2a version of this antibody was also used for the immunohistochemical stainings of frozen tissue microarray (TMA) containing 10 invasive ductal carcinomas of the breast, 10 colorectal adenocarcinomas and 10 non-small cell lung cancers (US20200079873). |
Antibody First Published | Bacac et al. Anti-fap antibodies and methods of use. US20200079873 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
