Key features and details | |
Cat. No. | MABL-1558 |
Name | Anti-FAK mAbs |
Clone No. | AFD- 3C4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody is specific for chicken tensin. Tensin may be involved in cell migration, fibrillar adhesion formation, cartilage development and in linking signal transduction pathways to the cytoskeleton. |
Alternative Name | tensin; FAK; p210 |
UniProt | Q04205 |
Immunogen | The mouse version of this antibody was generated by immunizing mice with a mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src, and recognizes FAK. |
Application Notes | To characterise the binding of the mouse version of this antibody to tyrosine- phosphorylated protein, immunoblotting on tyrosine-phosphorylated proteins from CEFs, NIH 3T3, Rat-1, HOS and 10W was performed. Furthermore, immunoprecipitating on the same samples was preformed. The antibody showed a reaction in both experiments and has cross-reactivity (Kanner et al, 1990; pmid:2110361). Cell lysates from CE cells infected with RCAS/RCAS-FRNK/RCAS-FAK were immunoprecipitated using the mouse version of this antibody. Furthermore, the immunoprecipitated proteins are western blotted using this antibody (Richardson and Parsons, 1996; pmid:8606775). |
Antibody First Published | Kanner et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc Natl Acad Sci U S A. 1990 May;87(9):3328-32. PMID:2110361 |
Note on publication | To study transformation-relevant tyrosine kinase substrates, we have generated monoclonal antibodies to individual tyrosine phosphoproteins, including p130, p120, p110, and five additional phosphoproteins (p210, p125, p118, p85, and p185/p64). These antibodies detected several of the same tyrosine phosphoproteins in chicken embryo fibroblasts transformed by avian retroviruses Y73 and CT10, encoding the yes and crk oncogenes, respectively. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
