Anti-estrogen receptor beta mAbs

Cat. No.

MABL-1533

Name

Anti-estrogen receptor beta mAbs

Clone No.

AFD- CO1531

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human estrogen   receptor. The estrogen receptor is a nuclear hormone receptor. Binds   estrogens with an affinity similar to that of ESR1, and activates expression   of reporter genes containing estrogen response elements (ERE) in an   estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand   binding ability and has no or only very low ere binding activity resulting in   the loss of ligand-dependent transactivation ability.

Alternative Name

Estrogen receptor beta; ESR2; ER-beta;   Nuclear receptor subfamily 3 group A member 2

UniProt

Q92731

Immunogen

This antibody was raised by immunizing a rat   with human estrogen protein.

Application Notes

The antibody was used for western blot on   tissues of C57BL/6J wild-type mice (Montani et al, 2008; pmid:18281260).   Whole-cell extracts from cells expressing ERα-LBD or full-length ERβ were   immunoprecipitated with this antibody (Leong et al, 2005; pmid:16051668).

Antibody First   Published

Leong et al. Recruitment of histone   deacetylase 4 to the N-terminal region of estrogen receptor alpha Mol   Endocrinol. 2005 Dec;19(12):2930-42. PMID:16051668

Note on publication

ERα N-terminal-specific coregulators were   identified, a breast cDNA library was screened by T7 phage display and   isolated histone deacetylase 4 (HDAC4).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.