Key features and details | |
Cat. No. | MABL-1532 |
Name | Anti-estrogen receptor alpha mAbs |
Clone No. | AFD- H222.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IP, WB, ELISA, IHC |
Species Reactivity | Chicken, Cow, Monkey, Rat, red-sided garter snake, Human |
Basic Information | |
Specificity | This antibody is specific for human estrogen receptor. The estrogen receptor is a nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c- Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. |
Alternative Name | Estrogen receptor; ER; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; ESR1 |
UniProt | P03372 |
Immunogen | This antibody was raised by immunizing a Lewis rat, with affinity-purified estrophilin. |
Application Notes | ELISA was preformed on human breast cancer tissue,human,monkey and calf uterus tissue and hen oviduct tissue. For this ELISA this antibody was used (Greene et al, 1984; pmid:6200700). Semi-confluent HeLa cells (3-5 x 106 cells/9 cm Petri dish) transfected with 10 ug of recombinant DNA (HEO- HE14) were immunoprecipitated with this antibody (Kumar et al, 1986; pmid:3780678).Immunohistochemistry was preformed, to compare them to their own antibodies, on human breast cancers using this antibody (Saati et al , 1993; pmid:8406995). Western blot was preformed on protein extracts from (i) transformed E. coli; (ii) COS cells transfected with pSG5-HGO or pSG5-HG19 (containing a deletion mutant of the ER cDNA lacking the sequence coding for the 178 N terminal amino-acids) the human breast- cancer cell line MCF-7 (ATCC HTB 22, Rockville, MD) and (iv) endometrial tissue. In this western blotting this antibody was used (Saati et al , 1993; pmid:8406995). |
Antibody First Published | Greene et al. Immunochemical studies of estrogen receptors J Steroid Biochem. 1984 Jan;20(1):51-6. PMID:6200700 |
Note on publication | Describes a Lewis rat being immunized, with affinity-purified estrophilin, to create this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
