Key features and details | |
Cat. No. | MABL-1509 |
Name | Anti-EPHA3 mAbs |
Clone No. | AFD- FA225-C10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for EPHA3. |
Alternative Name | Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; Human embryo kinase; HEK; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1 |
UniProt | P29320 |
Immunogen | The original mouse parental antibody IIIA4 was generated by intraperitoneal immunization of BALB/c mice with LK63 cells. This antibody was synthetically engineered to mimic a binding specificity of the original IIIA4, by grafting the CDRs of the parental antibody onto human framework segments. |
Application Notes | This antibody is a humanized affinity improved version of the original mouse parental antibody IIIA4. The binding affinity of this antibody for EphA3 antigen was determined by ELISA. Flow cytometry was performed with this antibody on cells from patients with multiple myeloma (US20110243934). In the xenograft model of leukemia the antibody directly affects the tumor cells with no effect on stromal and vascular elements. In human tumor xenografts of prostate cell line DU-145 in nude mice, FA225-C10 antibody produced a significant inhibition of tumor growth. In this model the tumor cells do not express EphA3 but the tumor stroma and neovasculature express EphA3 and treatment with antibody reduced both tumor stroma and new vessel density (Charmsaz et al., 2014). |
Antibody First Published | Varghese et al. Epha3 antibodies for the treatment of multiple myeloma. US20110243934 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
