Key features and details | |
Cat. No. | MABL-1503 |
Name | Anti-EpCAM mAbs |
Clone No. | AFD- KS1/4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Immunoperoxidase staining, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds to the EpCAM receptor which is expressed on surface of adenocarcinomas and almost all epithelial cell membranes except mesodermal and neural cell membranes. |
Alternative Name | CD326; Ep-CAM; KS1/4 antigen; KSA; Epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; 17-1A-antigen; EGP314; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1. |
UniProt | P16422 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human adenocarcinoma of the lung cell line UCLA P3. |
Application Notes | This antibody has been shown to be an effective agent for the invitro detection of disease and invivo diagnosis, prognosis and treatment of adenocarcinoma. Initial specificity of the antibody was determined by ELISA. Immunoperoxidase staining and immunofluorescence staining techniques were used to identify the reactivity of the antibody with various tissues (PMID: 6362852). The monoclonal antibody-vinca alkaloid conjugate, KS1/4-DAVLB (LY256787) were administered to nude mice bearing P3/UCLA human lung adenocarcinoma tumors. It was shown that KS1/4 monoclonal antibody targets DAVLB to the P3/UCLA human lung adenocarcinoma in vivo in the human xenograft model and that an increased therapeutic index may be achieved with LY256787 over conventional free drug therapy. (PMID: 3494841). KS1/4-DAVLB conjugates were also used for site directed therapy of epithelial malignancies (PMID: 2979063). |
Antibody First Published | Varki et al. Antigens Associated with a Human Lung Adenocarcinoma Defined by Monoclonal Antibodies. Cancer Research (1984); Vol- 44; 681-687. PMID:6362852 |
Note on publication | Describes the generation and characterization of antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
