Key features and details | |
Cat. No. | MABL-1502 |
Name | Anti-EpCAM mAbs |
Clone No. | AFD- HEA125 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, RIA, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the extracytoplasmic part of human EpCAM, a stable and widely- distributed marker expressed on the surface of many adenocarcinomas. It reacts with carcinomas derived from the colorectum, stomach, pancreas, liver, lung, mammary gland, ovary, thyroid, kidney, urinary bladder and prostate. |
Alternative Name | CD326; Ep-CAM; Epithelial cell adhesion molecule; Adenocarcinoma- associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor- associated protein GA733-2; Tumor-associated calcium signal transducer 1; HEA 125 |
UniProt | P16422 |
Immunogen | This antibody was raised by immunising BALB/c mice with the human colon carcinoma cell line HT-29. |
Application Notes | This antibody has applications in the detection of normal and neoplastic epithelial cells, and distinguishing carcinomas from non-epithelial neoplasms (Momburg et al, 1987). This antibody has been used in radioimmunoassays on live cells and immunoenzymatic staining assays on glutaraldehyde-fixed cells, as well as in immunoperoxidase staining of frozen and formalin-fixed paraffin tissue sections (Moldenhauer et al, 1987; Momburg et al, 1987). These revealed it to react with most normal human epithelial cells, but not non-epithelial tissue, and to intensely react with human carcinomas and metastatic lesions, but not lymphoma, melanoma, sarcoma or neuroblastoma cell lines (Moldenhauer et al, 1987; Momburg et al, 1987). Squamous cell carcinomas stain less strongly than adenocarcinomas, and keratinizing areas of tumor masses remain negative (Momburg et al, 1987). |
Antibody First Published | Moldenhauer et al. Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br J Cancer. 1987 Dec; 56(6):714–721. PMID:2449234 |
Note on publication | Describes the original generation of this antibody, and its characterisation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
