Key features and details | |
Cat. No. | MABL-1495 |
Name | Anti-Envelope protein DIII mAbs |
Clone No. | AFD- 2D73 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation, ELISA |
Species Reactivity | DENV |
Basic Information | |
Specificity | 2D73 reacts with residues 309-319 (DKEMAETQHGT) within domain III of the DENV envelope protein, shown crystallographically (Li et al, unpublished). Although raised against DENV-3 EDIII, 2D72 cross- reacts with EDIII from all four DENV serotypes. 2D73 does not react with other DENV-related flaviviruses, such as WNV, YFV and JEV. |
Alternative Name | DENV E DIII; Dengue virus envelope protein domain III; DENV envelope protein domain III; 2D-73 |
UniProt | |
Immunogen | BALB/c mice were immunised with recombinantly-produced envelope protein domain III (EDIII) from DENV-3. Splenocytes were obtained from immunised mice and fused with the NS-1 myeloma cell line to generate hybridomas. |
Application Notes | 2D73 was able to strongly neutralise DENV infection in a serotype-independent manner, shown using both ELISAs and ELISPOT-based assays (Li et al, 2013). 2D73 has also been used, as a Fab fragment, to crystallise the DENV-4 EDIII protein (Li et al, unpublished). |
Antibody First Published | Li et al. Dengue virus envelope domain III immunization elicits predominantly cross-reactive, poorly neutralizing antibodies localized to the AB loop: implications for dengue vaccine design. J Gen Virol. 2013 Oct;94(Pt 10):23851440 |
Note on publication | Describes the generation and characterisation of 25 cross-reactive anti-DENV mAbs. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
