Key features and details | |
Cat. No. | MABL-1494 |
Name | Anti-Envelope protein (soluble) mAbs |
Clone No. | AFD- Z3L1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation, NTRL, SPR, ELISA, FC |
Species Reactivity | Zika Virus |
Basic Information | |
Specificity | Z3L1 binds to the ZIKV soluble envelope (sE) protein, shown using SPR, kinetic competition assays and crystallography (Wang et al, 2016). This mAb is highly specific for ZIKV, and was shown to exhibit no neutralisation activity against DENV-1 to DENV-4 virions, which are highly related to ZIKV. It was shown crystallographically that the Z3L1 Fab region binds to domain I and domain II of sE. ZIKV has recently become a major global health concern, following a 2015 outbreak. Viral infection is associated with neurological complications and microencephaly. |
Alternative Name | ZIKV sE; Zika virus soluble envelope protein; Zika virus envelope protein; ZIKV soluble envelope protein; ZIKV envelope protein; Zika virus sE protein; ZIKV sE protein; Z3-L1 |
UniProt | |
Immunogen | Anti-ZIKV mAbs were obtained from the sera and peripheral blood mononuclear cells of a ZIKV-infected patient. |
Application Notes | Z3L1 was confirmed to bind ZIKV sE protein by ELISA, with an affinity of 5.39 uM shown by SPR (Wang et al, 2016). Using a FACS-based assay, it was shown that Z3L1 was sufficient for complete neutralisation of viral activity against infected C6/36 and Vero cells. In vivo, it has been shown that Z3L1 protected IFN receptor KO mice from ZIKV infection. |
Antibody First Published | Wang et al. Molecular determinants of human neutralizing antibodies isolated from a patient infected with Zika virus. Sci Transl Med. 2016 Dec 14;8(369):369ra179. PMID:27974667 |
Note on publication | Describes the identification of several anti-ZIKV mAbs from the blood of a patient with ZIKV infection. The mAbs identified were characterised for antigen specificity using biochemical and biophysical methods, and a few of the mAbs found were crystallised with the ZIKV sE protein. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
