Key features and details | |
Cat. No. | MABL-1488 |
Name | Anti-Endosialin mAbs |
Clone No. | AFD- Clone 8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds mouse endosialin. |
Alternative Name | CD248; TEM1; Tumor endothelial marker 1 |
UniProt | Q91V98 |
Immunogen | The original antibody was generated by immunizing New Zealand white rabbits with soluble mouse endosialin extracellular domain-mouse Fc fusion protein. |
Application Notes | The binding specificity of this antibody towards mouse endosialin was checked with ELISA (US20100260769). This antibody was used for the immunofluorescent staining of mature blood vessels of normal lung in the wild type mice and huCD248 knock-in mice. It was able to detect both mouse and human CD248 (PMID: 26327620). |
Antibody First Published | Rybinski et al. Targeting endosialin/CD248 through antibody-mediated internalization results in impaired pericyte maturation and dysfunctional tumor microvasculature. Oncotarget. (2015); 6(28): 25429–25440. PMID:26327620 |
Note on publication | Describes the use of this antibody for immunostaining of CD248 and internalization assays of MORAb-004. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
