Cat. No.
MABL-3227
Application
flow cytometry, WB, IF
Isotype
Engineer antibody
Species Reactivity
Vesicular stomatitis Indiana virus
Clone No.
8G5F11
From
Recombinant Antibody
Specificity
This antibody recognises vesicular stomatitis Indiana virus Glycoprotein (VSV-Ind G) (also shows activity against VSV-G pseudotyped lentiviruses).
Alternative Names
vesicular stomatitis virus glycoprotein; glycoprotein; Glycoprotein; vcv- glycoprotein; vsv; vesicular stomatitis virus; I1; G; G protein; G-protein; vesicular stomatitis Indiana virus; vesicular stomatitis Indiana virus Glycoprotein; VSV-Ind G
UniProt
Immunogen
The antibody was raised by immunizing mice with VSV.
Application Notes
FC: The antibody was successfully used in flow cytometry with HEK-293T cells transfected with the G protein gene (Nikolic et al., 2018). IF: 8G5F11 antibody was used to stain cell-surface VSVG proteins in HeLa cells which had been previously transfected with VSVG-EYFP in an experiment on cellular transport inhibition (Yonemura et al., 2016). IF: Xing et al. (2016) detected VSVG in multiple immunofluorescence experiments using 8G5F11 antibody.
Antibody First Published
Lefrancois and Lyles The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology. 1982 Aug;121(1):157-67. PMID:18638751
Note on publication
The article describes the method of generation of various antibodies against surface glycoprotein of two serotypes of the VSV: Indiana ans New Jersey. Among them there is the 8G5F11 (I1) antibody. The paper further characterises those antbodies and analyses their epitopes in relation to one another.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
