Cat. No.
MABL-3906
Application
ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
10E 4
From
Recombinant Antibody
Specificity
This antibody specifically binds amino acids 'KEVFDN' in the extracellular loop 2 domain (CD9- ECL2) of human CD9. This protein is an intergral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Extracellular vesicles (EVs) such as exosomes are important mediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins like CD9. EVs have strong potential as blood-based or urine-based biomarkers for the diagnosis, prognostication, and surveillance of cancer.
Alternative Names
p24; MIC3; MRP-1; GIG2; Tspan-29; TSPAN29; 5H9 antigen; Cell growth- inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; Tetraspanin-29
UniProt
P21926
Immunogen
The original antibody was generated by immunizing an alpaca with a CD9-LaFc recombinant fusion protein containing human CD9 (Ser112-lle195) and alpaca LaFc sequence. The peripheral blood lyphocytes from the alpaca were used to generate a phage display library and the actual clone was isolated by panning the library against human CD9-his protein.
Application Notes
The binding specificity of this antibody for recombinant his tagged CD9 protein was determined using ELISA (CN117659191).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
