Rabbit anti-VAT (Vesicular Acetylcholine Transporter) Antibody

Name

Rabbit anti-VAT (Vesicular Acetylcholine Transporter) Antibody

中文名字

兔抗VAT抗体; 兔抗囊泡乙酰胆碱转运蛋白抗体

Description

The antibody produces strong labeling of VAChT at a dilution of 1/3,000 – 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Human, Mouse, Rat

Preabsorption Control

/

Alternate Names

Solute carrier family 18, member 3; solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3; rVAT; VACht, anti-VAT, anti-VACht

RRID

AB_572269

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Slc18a3

Entrez Gene ID

Entrez Gene: 6572      Human
Entrez Gene: 20508    Mouse
Entrez Gene: 287721   Rat

NCBI Gene Aliases

/

Sequence

Q16572

APPLICATION

Quality Control

The antibody produces significant indirect immunofluorescence staining and significant biotin-avidin/HRP staining at dilutions of 1/5,000–1/10,000 of VAChT in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat VAT (VAChT) (511–530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.

Tissue

Rat basal forebrain

Perfusion Fixation

• Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use IF or Bn-AV/HRP according to manufacturer’s directions

Suggested Dilution

 1/5,000 –1/10,000 in PBS/0.3% Triton X-100 - Bn-AV/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles