Rabbit anti-Vasopressin Antibody

Name

Rabbit anti-Vasopressin Antibody

中文名字

兔抗血管加压素抗体

Description

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 – 1/4000 dilution in rat hypothalamus.
Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Anole, Bluehead Wrasses (Fish), Chicken, Clarias Batrachus (Catfish), Cockroach, Ewe / Sheep, Hamster, Human, Hydra Vulgaris (Polyp), Locust, Mole Rat, Monkey, Mouse, Pigeon, Rat, Shrew, Squirrel

Preabsorption Control

/

Alternate Names

Vasopressin (Arg); Antidiuretic Hormone; Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; ARG-vasopressin; prepro-AVP-NPII; neurohypophyseal; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; AVP-NPII; VP; ADH; ARVP; AVRP, anti-vasopressin

RRID

AB_572219

Immunogen

This information is proprietary to Mabioway

Gene Symbol

 AVP

Entrez Gene ID

Entrez Gene: 396101         Chicken
Entrez Gene: 443477         Ewe / Sheep
Entrez Gene: 551               Human
Entrez Gene: 101717720    Mole Rat
Entrez Gene: 717101         Monkey
Entrez Gene: 11998           Mouse
Entrez Gene: 24221           Rat
Entrez Gene: 102870182    Shrew

NCBI Gene Aliases

/

Sequence

11000-17-2

APPLICATION

Quality Control

The antibody produces significant fluorescent staining and significant biotin-avidin/HRP staining at a 1/2,000 – 1/4,000 dilution in rat hypothalamus. Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Preadsorption with as much as 100 mg/mL of oxytocin had no effect on immunolabeling

Tissue

Rat paraventricular nucleus and supraoptic nucleus

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by manufacturers.

Suggested Dilution

1/2,000 – 1/4,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles