Rabbit anti-Vesicular Inhibitory Amino Acid Transporter (VIAAT) Antibody

Name

Rabbit anti-Vesicular Inhibitory Amino Acid Transporter (VIAAT) Antibody

中文名字

兔抗水泡抑制性氨基酸转运蛋白抗体； 兔抗VIAAT抗体

Description

The ImmunoStar VIAAT antiserum was quality control tested using standard immunohistochemical methods in rat brain and spinal cord using biotin/avidin-HRP techniques.Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VIAAT peptide residues 511-525.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

Solute carrier family 32 member 1; GABA and glycine transporter; Vesicular GABA transporter; Vesicular inhibitory amino acid transporter; solute carrier family 32 (GABA vesicular transporter), member 1; VGAT / SLC32A1; rGVAT; Vgat, anti-Vgat, anti-VIAAT

RRID

AB_10013882

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Slc32a1

Entrez Gene ID

Entrez Gene: 83612      Rat

NCBI Gene Aliases

/

Sequence

Q9H598

APPLICATION

Quality Control

The VIAAT antiserum was quality control tested using standard immunohistochemical methods in rat brain and spinal cord using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble preadsorption and western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VIAAT peptide residues (511–525). Western blot analysis of immunoprecipitated rat brain homogenates demonstrates a dense immunoreactive band of approximately 57 kD and a minor band of approximately 36 kD.

Tissue

Rat brain and spinal cord

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hours at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component in fixation. If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Rat VIAAT (511–525) 25 mg/mL diluted antibody completely eliminates immunolabeling

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/AV-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/5,000–1/10,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles