Cat. No | MBA-050 |
Name | Mouse anti-Tyrosine Hydroxylase Antibody |
中文名字 | 兔抗酪氨酸羟化酶抗体 |
Description | The monoclonal Tyrosine Hydroxylase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000 – 1/8000 with the biotin/avidin-HRP technique. This antibody has been used successfully for IHC, ICC, FC, and IP. Western Blot: This antibody does not cross react with dihydropterdine reductase, dopamine-B-hydroxylase, phenylethanolamine-N-methyltransferase, phenylalanine hydroxylase or tryptophan hydroxylase using Western Blot methods. |
Clonality | monoclonal |
Isotype | IgG |
Host | Mouse |
Quantity/Volume | 50ul/100 µL |
State | antibody |
Reacts With | Alpaca (Llama), Amphibian, Anuran (Frog), Anuran (Urodele), Aplysia Californica (Sea Slug), Bee, Bird, Blowfly, Boar, Budgerigars, Canary, Cat, Chick, Chicken, Clam, Cockroach, Cod (Fish), Crab, Crayfish, Dog, Drosophila (Fly), Eel, Emu, Enegal Bichirs (Eel), Ewe, Ferret, Fish, Fly, Fox, Frog (Xenopus Laevis), Fruit Fly, Gastropod (Slug), Gastropoda, Gecko, Gerbil, Goldfish, Guinea Pig, Guppies, Hamster (Mesocricetus Auratus), Helisoma Duryi (Snail), Hen, Human, Iguana, Ilyanassa Obsoleta (Sea Snail), Lamprey, Leech, Lizard, Lobster, Locust, Mexican Axolotl (Salamander), Mollusca, Monkey (Macaca Fascicularis), Mouse, Newt, Opossum, Parrot, Periplaneta Americana (Cockroach), Phestilla Sibogae (Sea Coral), Phestilla Sibogae (Sea Slug), Phoronis Pallida (Phoronida), Pig, Pigeon, Possum, Prawn, Quail, Rabbit, Rat, Renilla Koellikeri (Sea Pansy), Rodent, Salamander, Salmon, Sea Fish, Sea Slug, Shark, Sheep, Short-Beaked Echidna, Shrew, Snail, Snake, Sparrow, Spisula (Clam), Squirrel Monkey, Starling, Stickleback, Tadpole, Trout, Turkey, Turtle, Urodele Amphibian, Water Buffalo, Worm, Yeast, Zebra Finch, Zebrafish |
Preabsorption Control | / |
Alternate Names | Tyrosine 3-monooxygenase (TH); TYH; Tyrosine 3-hydroxylase, anti-TH, anti-tyrosine hydroxylase |
RRID | |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | Th |
Entrez Gene ID | Entrez Gene: 408930 Bee |
NCBI Gene Aliases | / |
Sequence | P78428 |
APPLICATION | |
Quality Control | The antibody has wide species cross-reactivity because it recognizes an epitope in the catalytic core of the TH molecule where extensive species homology exists. TH antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. This antibody does not cross react with phenylalanine hydroxylase or tryptophan hydroxylase. The immunogen used to produce the TH antibody was full-length TH purified to homogeneity from PC12 cells, which are of rat origin. The exact epitope recognized by this antibody is not known but our studies indicate that following partial digestion of TH with the protease chymotrypsin the antibody recognizes the 34 kDa catalytic core of TH. HEK293 cells were transiently transfected to express human TH protein. Protein extracts from non-transfected and transfected cells were resolved by SDS-PAGE and transferred to nitrocellulose for western blotting. The blot was probed using our monoclonal antibody to TH and then stripped and re-probed using an antibody to beta-actin. This analysis shows that only extracts derived from cells transfected to express TH contain TH protein identified by the 60 kDa immunoreactive band, while non-transfected and transfected cells both express beta-actin protein. |
Tissue | Rat striatum and substantia nigra |
Perfusion Fixation | • Fixture: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. |
Absorption Control | |
Sections | 10 µm cryostat or 50 µm vibratome |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by the manufacturers. |
Suggested Dilution | 1/4,000 – 1/8,000 in PBS/0.3% Triton X-100 Bn-Av/HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles |
