Rabbit anti-DBH (Dopamine-beta-Hydroxylase) Antibody

Name

Rabbit anti-DBH (Dopamine-beta-Hydroxylase) Antibody

中文名字

兔抗DBH抗体;兔抗多巴胺β羟化酶抗体

Description

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 – 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla.
The above image is of DBH positive neurons in rat brainstem, vibratome sectioned, ABC / DAB detection.
Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

 Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch

Preabsorption Control

/

Alternate Names

DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH

RRID

AB_572229

Immunogen

This information is proprietary to Mabioway

Gene Symbol

DBH

Entrez Gene ID

Entrez Gene:  101082957   Cat
Entrez Gene:  101687995   Ferrett
Entrez Gene:  102038113   Finch
Entrez Gene:  100727873   Guuinea Pag
Entrez Gene:  100761411   Hamster
Entrez Gene:  1621            Human
Entrez Gene:  574105        Monkey
Entrez Gene:  13166          Mouse
Entrez Gene:  733609        Pig
Entrez Gene:  107321868  Quail
Entrez Gene: 25699           Rat
Entrez Gene: 102067155   Sparrow
Entrez Gene: 106851290   Starling
Entrez Gene:  280758       Steer (Cattle)
Entrez Gene: 100544779   Turkey
Entrez Gene: 102455557   Turtle
Entrez Gene: 100224356   Zebra Finch (Bird)

NCBI Gene Aliases

/

Sequence

P09172

APPLICATION

Quality Control

The antibody has significant indirect immunofluorescence at 1/400 –1/800 and significant biotinstreptavidin/HRP staining at a 1/2,000–1/4,000 dilution in rat brain stem, cerebellum and adrenal medulla. Using western blot of purified DBH the antiserum detects a triplet at approximately 72–74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Tissue

Rat brainstem, cerebellum and adrenal medulla

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldephyde in 0.1 M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjugation with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn-SA/HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/2,000–1/4,000 in PBS/0.3% Triton X-100 – Bn-SA/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.