Cat. No | MBA-022 |
Name | Rabbit anti-FMRF-amide (Cardio-excitatory Peptide) Antibody |
中文名字 | 兔抗FMRF酰胺抗体;兔抗心脏兴奋肽抗体 |
Description | The ImmunoStar FMRF-Amide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Abalone (Sea Snail), Acoela (Flatworm), Annelida (Segmented Worm), Anthomedusae, Aplysia (Sea Slug), Aplysia Californica (Sea Slug), Bat, Beetle, Brachiopods, Brown Trout (Fish), Bullfrog, Caenogastropod, Caenogastropod, Carausius Morosus (Insect), Carp (Fish), Catfish, Centipede, Chiton, Cichlid Fish, Clione Limacina Pteropod (Fruit Bat), Cockroach, Crab, Crayfish, Crocodile, Crustacean, Decapod Crustacea, Dermophis Mexicanus Mexican Caecilian (Amphibian ), Diploptera Punctata (Beetle Cockroach), Earwig / forcep bug, Fish, Fly, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gastropod (Slug), Gerbil, Hagfish, Hagfish, Helix Pomatia (Snail), Hermit Crab, Human, Idotea Emarginata (Isopod), Ilyanassa Obsoleta (Sea Snail), Jellyfish, Lamprey, Leech, Lepidodasy, Lepismachilis Y-Signata, Lizard, Lobster, Locust, Locusta Migratoria (Locust), Lymnaea Stagnalis (Snail), Macrobiotus (Water Bear), Manduca Sexta (Moth), Marine, Marine Gastrotrichs , Micro-Organisms, Mollusc, Mollusca, Mollusk, Monkey, Mopalia Muscosa (Mossy Chiton), Moth, Mouse, Mussel, Mussel (Mytilus Trossulus), Octocorallia (Soft Coral), Phascolion Strombus (Sipuncula), Phestilla Sibogae (Sea Slug), Phyllodoce Maculata (Phyllodocidae), Planarian , Pleurobranchaea Japonica (Sea Slug), Polyplacophora (Chitons), Pseudaletia Unipuncta (Moth), Pygmy Squid, Ragworm, Rat, Ray, Rhodnius Prolixus (Bug), Salamander (Urodele), Sea Cucumber, Sea Pansy, Sea Slug, Shark, Shrew, Skark, Slug, Snail, Spadella Cephaloptera (Arrow Worm), Spionid Polychaete, Three-Spined Stickleback (Fish), Tick, Trout, Worm (Digenea), Worm (Eisenia Fetida), Worm (Lumbricus Terrestris), Zebrafish |
Preabsorption Control | / |
Alternate Names | Anti-FMRF |
RRID | AB_572232 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | LOC100533232 |
Entrez Gene ID | 100533232 |
NCBI Gene Aliases | / |
Sequence | 64190-70-1 |
APPLICATION | |
Quality Control | The FMRF-Amide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of FMRF-Amide. |
Tissue | Rat hypothalamus and spinal cord |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20–30 min. |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn/AV-HRP according to manufacturers’ directions. |
Suggested Dilution | 1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles. |
