Rabbit anti-CRF (Corticotropin Releasing Factor) Antibody

Name

Rabbit anti-CRF (Corticotropin Releasing Factor) Antibody

中文名字

兔抗CRF抗体; 兔抗促肾上腺皮质激素释放因子抗体

Description

The ImmunoStar antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1000 – 1/2000 in PBS/0.3% Triton X-100 – Biotin/Avidin-HRP Technique.
Immuno-labeling is completely abolished by preadsorption with synthetic CRF at 100 ug per mL of diluted antibody.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Mouse, Rat, Salmon, Turtle

Preabsorption Control

/

Alternate Names

Corticoliberin; Corticotropin-Releasing Hormone,Endorpholiberin; corticotropin releasing hormone, anti-CRF

RRID

AB_572228

Immunogen

This information is proprietary to Mabioway

Gene Symbol

CRH

Entrez Gene ID

Entrez Gene:  12917    Mouse
Entrez Gene:  81648    Rat

NCBI Gene Aliases

/

Sequence

P06850

APPLICATION

Quality Control

The CRF antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Immunolabeling is completely abolished by preadsorption with synthetic CRF at 100 mg per mL of diluted antibody.Requires animal pretreatment such as colchicine or adrenalectomy

Tissue

Rat median eminence

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1 –0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

CRF 100 µg/mL diluted serum

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/Av-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/1,000 –1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.