Cat. No | MBA-018 |
Name | Rabbit anti-CCK-8 (Cholecystokinin Octapeptide) Antibody |
中文名字 | 兔抗CCK-8（胆囊收缩素八肽）抗体 |
Description | The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Blowfly, Bubalus Bubalis (Water Buffalo), Cat, Chick, Decapod Crustacea, Gerbil, Guinea Pig, Hamster, Human, Lizard, Lobster, Mollusk, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Pigeon, Rabbit, Raccoon, Rat, Sheep, Snail, Snail (Bulla Gouldiana) , Sting Ray, Tree Frog, Trout, Zacco Platypus (Minnow) |
Preabsorption Control | / |
Alternate Names | Cholecystokinin-8; CCK, anti-CCK |
RRID | AB_572224 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | CCK |
Entrez Gene ID | Entrez Gene: 102415979 Bubalus Bubalis (Water Buffalo) |
NCBI Gene Aliases | / |
Sequence | 25126-32-3 |
APPLICATION | |
Quality Control | The antibody has significant indirect immunofluorescence staining at a 1/100–1/200 dilution and significant Biotin-avidin/HRP immunostaining at a 1/500-1/1,000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble preadsorption with the peptides in question at a final concentration of 10-6 M. CCK-8 immunolabeling was completely abolished by preadsorption with CCK-8, gastrin 17 and gastrin 34. Preadsorption with the following peptides resulted in no reduction of immunostaining: aCGRP, bCGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide. |
Tissue | Rat hypothalamus, spinal cord |
Perfusion Fixation | • Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20–30 min. |
Absorption Control | / |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use IF or Bn/AV-HRP according to manufacturer’s directions. |
Suggested Dilution | 1/2,000 – 1/8,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles. |
