Rabbit anti-Calretinin Antibody

Name

Rabbit anti-Calretinin Antibody

中文名字

兔抗钙视网膜蛋白抗体

Description

The antibody has a proven maximum biotin-avidin/HRP staining at a 1/1000 – 1/4000 dilution in rat cortex, hippocampus and hypothalamus.
The antiserum has been characterized as specific to calretinin; please see references listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

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Alternate Names

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RRID

AB_572223

Immunogen

This information is proprietary to Mabioway

Gene Symbol

CALB2

Entrez Gene ID

Entrez Gene: 117059  Rat

NCBI Gene Aliases

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Sequence

>sp|P22676|CALB2_HUMAN Calretinin OS=Homo sapiens

APPLICATION

Quality Control

The antibody has significant biotin-avidin/HRP staining at a 1/1,000–1/4,000 dilution in rat cortex, hippocampus and hypothalamus. The antiserum has been characterized as specific to calretinin; please see references listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Tissue

Rat cortex, hippocampus, and hypothalamus

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

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Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Bn-Av/HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/1,000–1/4,000 in PBS/0.3% Triton X-100 - Bn-Av/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.