Rabbit anti-Calbindin D-28 Antibody

Name

Rabbit anti-Calbindin D-28 Antibody

中文名字

兔抗钙结合蛋白D-28抗体

Description

The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/5,000 – 1/10,000 dilution in rat striatum, cortex, and hippocampus.
The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Mouse, Rat

Preabsorption Control

/

Alternate Names

Calbindin; nCAB27; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein; Vitamin D-dependent calcium-binding protein, avian-type; CALB1; calbindin 1, 28kDa, anti-Calbindin

RRID

AB_572222

Immunogen

This information is proprietary to Mabioway

Gene Symbol

CALB1

Entrez Gene ID

Entrez Gene: 12307  Mouse
Entrez Gene: 83839  Rat

NCBI Gene Aliases

MGC13895

Sequence

>sp|P05937|CALB1_HUMAN Calbindin OS=Homo sapiens

APPLICATION

Quality Control

The antibody has significant biotin-avidin/HRP and indirect immunofluorescence staining at a 1/10,000 – 1/15,000 dilution in rat striatum, cortex, and hippocampus. The antiserum has been characterized as specific to calbindin D-28k; please see reference listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Tissue

Rat striatum, hippocampus, and cortex

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
 • Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

/

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/Av-HRP according to manufacturer’s directions

Suggested Dilution

1/10,000–1/15,000 in PBS/0.3% Triton X-100 - Bn-SA/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.