Rabbit anti-ACTH (Adrenocorticotropic Hormone) Antibody

Name

Rabbit anti-ACTH (Adrenocorticotropic Hormone) Antibody

中文名字

兔抗促肾上腺皮质激素抗体

Description

The antibody produces a maximum biotin-streptavidin/HRP staining at a 1/500 – 1/1000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The recommened dilution series is 1/500-1/1000 in PBS/0.3% Triton X-100 – Bn-SA/HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of ACTH.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Dove, Eel, Goldfish, Hamster, Human, Monkey, Mouse, Ram (Sheep), Rat, Trout

Preabsorption Control

/

Alternate Names

anti-ACTH

RRID

AB_572216

Immunogen

This information is proprietary to Mabioway

Gene Symbol

POMC

Entrez Gene ID

Entrez Gene: 102092785  Dove
Entrez Gene: 100756311  Hamster
Entrez Gene: 5443  Human
Entrez Gene: 694430  Monkey
Entrez Gene: 18976  Mouse
Entrez Gene: 443212  Ram (Sheep)
Entrez Gene: 24664  Rat
Entrez Gene: 100136771  Trout

NCBI Gene Aliases

ACTHR,MGC12579

Sequence

>sp|P01189|COLI_HUMAN Pro-opiomelanocortin OS=Homo sapiens

APPLICATION

Quality Control

The antibody produces significant fluorescein staining at a 1/100–1/200 dilution and significant biotin-avidin/HRP staining at a 1/2,000–1/4,000 dilution in rat anterior/intermediate pituitary. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Staining is completely eliminated by pretreatment of the diluted antibody with 100 mg/mL of ACTH.

Tissue

Rat anterior and intermediate pituitary

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

ACTH 100 µg/mL diluted serum

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use IF or Bn-avidin/HRP according to manufacturers’ directions.

Suggested Dilution

1/2,000–1/4,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles