Rabbit anti-Alpha-MSH (Melanocyte Stimulating Hormone) Antibody

Name

Rabbit anti-Alpha-MSH (Melanocyte Stimulating Hormone) Antibody

中文名字

兔抗α-MSH抗体

Description

The alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using biotin/avidin-HRP techniques.
Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Trout, Cat, Frog

Preabsorption Control

/

Alternate Names

POMC; proopiomelanocortin, anti-Alpha-MSH

RRID

AB_572218

Immunogen

This information is proprietary to Mabioway

Gene Symbol

POMC

Entrez Gene ID

Entrez Gene: 101087223  Cat
Entrez Gene: 496777  Frog (Xenopus Laevis)
Entrez Gene: 100720803  Guinea Pig
Entrez Gene: 100756311  Hamster
Entrez Gene: 5443  Human
Entrez Gene: 694430   Monkey
Entrez Gene: 18976   Mouse
Entrez Gene: 100344462   Rabbit
Entrez Gene: 24664  Rat
Entrez Gene: 100136771  Trout

NCBI Gene Aliases

/

Sequence

9002-79-3. SYSMEHFRWGKPV

APPLICATION

Quality Control

The alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat pituitary using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of α-MSH.

Tissue

Rat pituitary

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

α-MSH 100 µg/mL diluted serum

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use IF or Bn/AV-HRP according to manufacturers’ directions.

Suggested Dilution

1/4,000–1/6,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. Please read the instruction booklet carefully before beginning the procedure.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.