Rabbit anti-5-HTP (5-Hydroxytryptophan) Antibody

Name

Rabbit anti-5-HTP (5-Hydroxytryptophan) Antibody

中文名字

兔抗5-HTP（5-羟色氨酸）抗体

Description

The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 – 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA.
The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat, Sea Slug (Aplysia Californica, Tritonia Diomedea

Preabsorption Control

/

Alternate Names

anti-5-HTP

RRID

AB_572215

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Avp

Entrez Gene ID

24221

NCBI Gene Aliases

DI, Va

Sequence

4350-09-8

APPLICATION

Quality Control

The antibody has significant biotin-avidin/HRP staining at a 1/1,000–1/2,000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by preadsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by preadsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.

Tissue

Rat raphe nuclei

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
 • Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

/

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn-AV/HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Unopened vial store at 2°–8°C until expiration date