Rabbit anti-5-HT (Serotonin) Transporter Antibody

Name

Rabbit anti-5-HT (Serotonin) Transporter Antibody

中文名字

兔抗5-HT（血清素）转运蛋白抗体

Description

The serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/10,000 – 1/15,000 in PBS/0.3% Triton X-100 – Biotin/avidin-HRP.
By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The predicted molecular weight of serotonin transporter based on its nucleic acid sequence is 67,815.  The actual weight that one might observe from tissue extracts could be higher due to glycosylation.  We validated the antibody with rat SERT overexpressed in an insect cell expression system.  The MW from that was about 67 kD.  Our SERT antibody dilution was 1:20,000 using chemiluminescent detection.
Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Human, Marine, Monkey, Mouse, Rat, Sparrow

Preabsorption Control

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Alternate Names

5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

RRID

AB_572209

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Slc6a4

Entrez Gene ID

Entrez Gene: 6532  Human
Entrez Gene: 15567  Mouse
Entrez Gene: 25553  Rat
Entrez Gene: 102068406  Sparrow

NCBI Gene Aliases

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Sequence

>sp|P31645|SC6A4_HUMAN Sodium-dependent serotonin transporter

APPLICATION

Quality Control

The Serotonin Transporter (5HTT) was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, specifically labels a single band. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT transporter (602–622).

Tissue

Rat raphe nuclei, hypothalamus, cortex, and spinal cord

Perfusion Fixation

• Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

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Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/Av-HRP reagents at dilutions recommended by the manufacturers.

Suggested Dilution

1/10,000 – 1/15,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Unopened vial store at 2°–8°C until expiration date