Rabbit anti-5-HT (Serotonin) Antibody

Name

Rabbit anti-5-HT (Serotonin) Antibody

中文名字

兔抗5-HT（血清素）抗体

Description

The serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/20,000-1/40,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 25 ug of serotonin/BSA.
Cross reactivity of Serotonin antisera was examined. With 5µg, 10µg and 25µg amounts the following substances did not react with Serotonin antisera diluted 1/20,000 using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Abalone (Haliotis Kamtschatkana) , Amphibian, Annelid Mushroom, Annelida (Segmented Worm), Ant, Ant (Pheidole Dentata), Antalis Entalis Scaphopoda, Aplysia (Sea Slug), Aplysia Californica (Sea Slug), Aplysia Punctata (Sea Slug), Arachnocampa (Gnat), Archidoris Pseudoargus (Sea Slug), Asterina Pectinifera (Starfish ), Astyanax Mexicanus (Cave Fish), Axolotl (Salamander), Bacteria, Beetle, Bird, Brachiopods, Bryozoans (Polyzoa), Buffalo, Bufo Marinus (Toad), Bug, Bumblebee, Butterfly, Butterfly (Monarch), Canine, Cat, Chick, Chicken, Chiton, Chrysoperla Carnea Green Lacewing , Clam, Clione Limacina Pteropod (Fruit Bat), Cockroach, Cod, Crab, Crayfish, Cricket, Crustaceans, Cupiennius Salei (Spider), Desert Locust, Dog, Dogfish (Shark), Dove, Drosophila (Fly), Drosophila Melanogaster (Fly), Feather Star, Ferret, Fish, Fish (Salmon), Flounder, Fly, Frog, Frog (Xenopus Laevis), Fruit Fly, Gastropod (Slug), Gastropod (Slug), Gerbil, Glowworm, Goldfish, Grasshopper, Guinea Pig, Hamster, Hedgehog, Helisoma Trivolvis (Snail), Hen, Hermit Crab, Honeybee, Human, Idiosepius Notoides (Squid), Ilyanassa Obsoleta (Sea Snail), Insect, Kitten (Cat), Lamprey, Leech, Lizard, Lobster, Lobster (Cycliophora), Lobster Larva, Locust, Lythrypnus Dalli (Fish), Macracantha (Cephalocarida), Manduca Sexta (Moth), Marmoset (Monkey), Mercenaria (Clam), Mexican salamander (Ambystoma  Mexicanum) , Mollusca, Mollusk, Monkey, Monkey (Macaque), Mosquito, Mosquito (Aedes Aegypti), Moth, Moth (Manduca Sexta), Mouse, Mudpuppy, Mussel, Newt, Nudibanch, Nudibranch Mollusc, Nudipleura, Octopus, Onychophora (Velvet Worm), Ovine (Sheep), Parhedyle Cryptophthalma (Sea Slug), Phoronis Pallida (Phoronida), Phyllodoce Maculata (Phyllodocidae), Phyllodoce Maculata (Polychaeta), Pig, Pigeon, Pilidium Larvae, Platynereis Dumerilii (Ragworm), Platypus, Pleurobranchaea Californica (Sea Slug), Pleurobranchaea Japonica (Sea Slug), Pleurodeles (Newt), Prawn, Praying Mantis, Ptychodera Flava (Worm), Quail, Rabbit, Raja Erinacea (Skate), Ram (Sheep), Rana Perezi (Frog), Rat, Red Abalone, Red Abalone, Renilla Koellikeri (Cnidarian ), Rhodnius Prolixus (Bug), Rhodnius Prolixus (Parasite), Salamander, Sand Dollar, Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Schistocerca Gregaria (Locust), Scorpaena Porcus (Fish), Scorpion, Scyliorhinus Canicula (Dogshark), Sea Bass (Fish), Sea Cucumber, Sea Lamprey, Sea Lily, Sea Scallop, Sea Slug, Sea Spider, Sea Star, Sea Urchin, Seahorse, Shark, Sheep, Shrew, Skate, Snail, Sparus Aurata (Fish), Spisula (Clam), Springtails, Squirrel Monkey, Sting Ray, Stomatopod Crustacean, Sturgon, Tapeworm, Tectura Scutum (Mollusk), Tick, Tigriopus Californicus (Copepod), Toad, Tragulus Javanicus (Mouse Deer), Tritonia Diomedea (Sea Slug), Trout, Turkey, Turtle, Worm, Worm (Digenea) , Worm (Diphyllobothrium Dentriticum) , Worm (Dugesia Tigrina) , Worm (Dugesia Tigrina), Worm (Phyllodoce Groenlandica), Worm (Polychaete Capitella), Zebra Finch (Bird), Zebrafish

Preabsorption Control

/

Alternate Names

anti-5-HT Rabbit

RRID

AB_572263

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Spl

Entrez Gene ID

104153

NCBI Gene Aliases

/

Sequence

50-67-9

APPLICATION

Quality Control

The Serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat hypothalamus, raphe nuclei and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of serotonin/BSA. Cross reactivity of Serotonin antisera was examined. With 5mg, 10mg and 25mg amounts the following substances did not react with diluted Serotonin antisera using the Bn-SA/HRP labeling method: 5-hydroxytryptophan, 5-hydroxyindole -3- acetic acid, and dopamine.

Tissue

Rat hypothalamus, raphe nuclei and spinal cord.

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

5-HT/BSA 25 ug/mL diluted serum

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or biotin/avidin-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/5,000-1/10,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Unopened vial store at 2°–8°C until expiration date