Goat anti-5-HT (Serotonin) Antibody

Name

Goat anti-5-HT (Serotonin) Goat Antibody

中文名字

山羊抗5-HT（血清素）抗体

Description

The serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/5000 – 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique.

Clonality

Polyclonal

Isotype

IgG

Host

goat

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Cat, Cod, Cow, Crayfish, Dolphin, Fish, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Mexican salamander (Ambystoma  Mexicanum), Monkey, Moth, Mouse, Pig, Rabbit, Ram (Sheep), Rat, Salamander, Snail, Tapeworm, Worm, Zebrafish

Preabsorption Control

/

Alternate Names

anti-5-HT Goat

RRID

AB_572262

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Spl

Entrez Gene ID

104153

NCBI Gene Aliases

/

Sequence

50-67-9

APPLICATION

Quality Control

The Serotonin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat hypothalamus and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg of serotonin/BSA conjugate per mL of diluted antiserum.

Tissue

Rat suprachiasmatic/hypothalamus and spinal cord.

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjugation with paraformaldehyde

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Cy3 or Bn/Av-HRP reagents at dilutions recommended by the manufacturers.

Suggested Dilution

1/5,000 –1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Unopened vial store at 2°–8°C until expiration date