Rabbit anti-5-HT (Serotonin) 7 Receptor Antibody

Name

Rabbit anti-5-HT (Serotonin) 7 Receptor Antibody

中文名字

兔抗-5-HT 7受体抗体，兔抗血清素抗体

Description

The 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 – 1/300 in PBS – biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies.Due to the difficulty with receptor antibodies, blot applications are not warranted and are included as specificity information only.
Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Cat, Grasshopper, Human, Hutchinsoniella Macracantha (Horseshoe Shrimp), Mouse, Pig, Rabbit, Rat

Preabsorption Control

/

Alternate Names

5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7, anti-5-HT 7

RRID

AB_572214

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Htr7

Entrez Gene ID

Entrez Gene: 101092146  Cat
Entrez Gene: 3363  Human
Entrez Gene: 15566  Mouse
Entrez Gene: 397158   Pig
Entrez Gene: 100009356  Rabbit
Entrez Gene: 65032   Rat

NCBI Gene Aliases

/

Sequence

>sp|P34969|5HT7R_HUMAN 5-hydroxytryptamine receptor 7

APPLICATION

Quality Control

The 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Due to the difficulty with receptor antibodies, blot applications are not warranted and are included as specificity information only. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C.

Tissue

Rat cortex and hippocampus

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.05M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Use glutaraldehyde (0.1–0.3%) in conjunction with paraformaldehyde.

Absorption Control

/

Sections

50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Bn/AV-HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/100–1/300 in PBS - Bn/Av-HRP technique
Note: use of Triton X-100 or other detergents is not recommended

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution
recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol
may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Unopened vial store at 2°–8°C until expiration date