Rabbit anti-5-HT (Serotonin) 6 Receptor Antibody

Name

Rabbit anti-5-HT (Serotonin) 6 Receptor Antibody

中文名字

兔抗-5-HT 6受体抗体，兔抗血清素抗体

Description

The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide.
Th 5HT 6-receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using indirect immunofluorescent and biotin/avidin-HRP techniques. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT6 receptor (CLERPPGTPRHPPGPPLW).
Immunolabeling of western blot revealed a single band of approximately 53kD.  Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Rat

Preabsorption Control

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Alternate Names

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RRID

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Immunogen

This information is proprietary to Mabioway

Gene Symbol

Htr6

Entrez Gene ID

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NCBI Gene Aliases

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Sequence

>sp|P50406|5HT6R_HUMAN 5-hydroxytryptamine receptor 6

APPLICATION

Quality Control

The 5HT 6-receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using indirect immunofluorescent and biotin/avidin-HRP techniques. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT6 receptor (CLERPPGTPRHPPGPPLW). Immunolabeling of western blot revealed a single band of approximately 53kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.

Tissue

Rat cortex, amygdala and hippocampus.

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.

Absorption Control

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Sections

50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Bn/Av-HRP at dilutions recommended by the manufacturer.

Suggested Dilution

1/500–1/1000 in PBS – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2°–8°C until expiration date.