Rabbit anti-5-HT (Serotonin) 5A Receptor Antibody

Name

Rabbit anti-5-HT (Serotonin) 5A Receptor Antibody

中文名字

兔抗-5-HT 5A受体抗体，兔抗血清素抗体

Description

The 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/200 – 1/400 in PBS/0.3% Triton X-100 – Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Clam, Rat

Preabsorption Control

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Alternate Names

5-hydroxytryptamine receptor 5A; 5-HT5A; REC17; 5ht5a,MR22; 5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled, anti-5-HT5a

RRID

AB_572213

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Htr5a

Entrez Gene ID

Entrez Gene: 25689  Rat

NCBI Gene Aliases

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Sequence

>sp|P47898|5HT5A_HUMAN 5-hydroxytryptamine receptor 5A

APPLICATION

Quality Control

The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only. Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17–34).

Tissue

Rat cortex and hippocampus

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

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Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Cy3 or Bn/AV-HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/100–1/300 in PBS/0.3% Triton X-100 – Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2°–8°C until expiration date.