Rabbit anti-Vesicular Monoamine Transporter 1 (VMAT) Antibody

Name

Rabbit anti-Vesicular Monoamine Transporter 1 (VMAT) Antibody

中文名字

兔抗泡状单胺转运蛋白1（VMAT）抗体； 兔抗VMAT抗体

Description

The VMAT1 antiserum was quality control tested using standard immunohistochemical methods in rat adrenal medulla using biotin/avidin-HRP techniques; the antiserum shows no reactivity in rat CNS.
Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT1 peptide residues 502-521.
Western blot analysis of immunoprecipitated rat adrenal homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

Chromaffin granule amine transporter; Solute carrier family 18 member 1; VAT1; VMAT-1; Vesicular amine transporter 1; Slc18a1. Cgat, Vmat1,Vmat-1; solute carrier family 18 (vesicular monoamine), member 1. Slc18a1,25693, anti-VMAT1

RRID

AB_10013883

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Slc18a1

Entrez Gene ID

Entrez Gene: 25693      Rat

NCBI Gene Aliases

/

Sequence

P54219

APPLICATION

Quality Control

The VMAT1 antiserum was quality control tested using standard immunohistochemical methods in rat adrenal medulla using biotin/avidin-HRP techniques; the antiserum shows no reactivity in rat CNS. Specificity of the antiserum was demonstrated by soluble preadsorption and western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT1 peptide residues (502–521). Western blot analysis of immunoprecipitated rat adrenal homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.

Tissue

Rat adrenal medulla

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hours at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component in fixation. If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Rat VMAT1 (502-521) 100 mg/mL diluted antibody completely eliminates immunolabeling

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Bn/AV-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/1,000 – 1/3,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles