Anti-Bovine coronavirus mabs [SF-3E4](MABL-3591)

Cat. No.

MABL-3591

Name

Anti-Bovine coronavirus mabs [SF-3E4](MABL-3591)

Clone No.

SF-3E4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralising, WB, ELISA

Species Reactivity

Bovinecoronavirus

Basic Information

Specificity

This antibody recognises an epitope in the S1 subunit of the BCV spike protein.

Alternative Name

BCV

UniProt

Immunogen

This antibody was isolated from an immunised   bovine scFv phage display library. The antibody repertoire of a Holstein Frisian   bull immunized against BCV was amplified, and the antibody- encoding cDNA   from isolated peripheral blood mononuclear cells was use

Application Notes

An scFv version of this antibody exhibits   good binding kinetics (Km = 595 pM) and neutralization ability (≥ 0.24 μg/ml) against BCV-V270 in ELISA analysis and   neutralisation assays using BCV-V270-infected VERO cell cultures (Fischer,   2017). Western blotting and a competition ELISA with BCV- V270 have shown   that this scFv recognises an epitope in the BCV spike protein (Fischer,   2017). In ELISA analysis, this scFv cross-reacts with BCV-L9 and the human   coronavirus strain OC43 (Fischer, 2017). This scFv is a potential therapeutic   candidate for the treatment of calf diarrhea caused by coronaviruses.

Antibody First   Published

Fischer Cytokines and immunoglobulin genetics in neonatal calf diarrhea Doctoral Dissertation, Faculty of   Veterinary Medicine, Justus Liebig University Giessen

Note on publication

Describes the original generation of this scFv, and its characterisation in ELISA and WB analysis

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.