Anti-SARS-CoV S glycoprotein mabs [256](MABL-3580)

Cat. No.

MABL-3580

Name

Anti-SARS-CoV S glycoprotein mabs 

Clone No.

256

From

Recombinant Antibody

Isotype

Engineer antibody

Application

NTRL, ELISA

Species Reactivity

SARSCoronavirus

Basic Information

Specificity

This antibody specifically binds the amino acids 472 and 480 in the S1 domain of the SARS- CoV Spike protein (Tor2 and GD03).

Alternative Name

Spike protein; S protein; SARS-CoV S   protein; S glycoprotein; E2; Peplomer protein; Spike protein S1

UniProt

P59594

Immunogen

The original antibody was produced by panning against GD03-RBD-C9, two non-immune human Ab phage display libraries and   neutralizing activity was tested against pseudotyped viruses.

Application Notes

This antibody inhibits the association of   SARS-CoV S protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the   virus. 256-IgG1 was capable of neutralizing both the mutations of the spike   protein at D480A and D480G. It also neutralized Tor2 and marginally   neutralized GD03. Neither of the antibodies 11A nor 80R competed with 256 IgG1 for binding to Tor2 suggesting that the binding epitope of 256 was different from that of 80R and 11A (Sui et. al, 2008)

Antibody First   Published

Sui et al. Broadening of neutralization   activity to directly block a dominant antibody-driven SARS-coronavirus   evolution pathway. PLoS Pathog. (2008); 4(11):e1000197. PMID:18989460

Note on publication

Describes the methods for generation of a broadly neutralizing antibody by using a combination of chain-shuffling as   well as hot-spot CDR mutagenesis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.