Anti-SARS-CoV S glycoprotein mabs [11A](MABL-3579)

Cat. No.

MABL-3579

Name

Anti-SARS-CoV S glycoprotein mabs [11A](MABL-3579)

Clone No.

11A

From

Recombinant Antibody

Isotype

Engineer antibody

Application

NTRL, ELISA

Species Reactivity

SARSCoronavirus

Basic Information

Specificity

This antibody specifically binds the amino acids 472 and 480 in the S1 domain of the SARS- CoV Spike protein (GD03).

Alternative Name

Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1

UniProt

P59594

Immunogen

The original antibody was produced by   panning against GD03-RBD-C9, two non-immune human Ab phage display libraries   and neutralizing activity was tested against pseudotyped viruses.

Application Notes

This antibody inhibits the association of   SARS-CoV S protein to the angiotensin- converting enzyme 2 (ACE2) receptor by   binding to specific amino acids on the S1 domain of the spike protein of the   virus strain GD03. 11A potently inhibited GD03-RBD’s binding to ACE2   expressing 293T cells. Kinetic analysis of 11A IgG binding to GD03-RBD   demonstrated high affinity interaction (KD = 2.260.7 nM). Single amino acid   changes at either position D480A or D480G resulted in complete loss of 11A   binding. (Sui et al., 2008)

Antibody First   Published

Sui et al. Broadening of neutralization activity to directly block a dominant antibody-driven SARS-coronavirus   evolution pathway. PLoS Pathog. (2008); 4(11):e1000197.PMID:18989460

Note on publication

Describes the methods for generation of a broadly neutralizing antibody by using a combination of chain-shuffling as   well as hot-spot CDR mutagenesis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.