Anti-Butyrylcholinesterase mAbs

Cat. No.

MABL-563

Name

Anti-Butyrylcholinesterase  mAbs

Clone No.

AFD- 11D8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

11D8 binds to both native and denatured   human butyrylcholinesterase (HuBChE) with KD ~0.26 nM (determined by ELISA - 0.9 nM by SPR). Binding of 11D8 excludes binding of mAb B2 18-5, suggesting   tht that bind to the same/similar epitope on huBChE. The antibody also binds   to human albumin, which can compete with huBChE for binding. huBChE in plasma is a serine esterase that catalyzes the hydrolysis of esters including   aspirin, cocaine, heroin, and octanoyl ghrelin.

Alternative Name

BChE

UniProt

P06276

Immunogen

11D8 wsa prepared by immunizing BChE -/- mice with sugar-diminished full-length recombinant human BChE (Hrabovska et al. 2010).

Application Notes

The percent active HuBChE bound to 11D8 in ELISAS is highest when the volume of plasma was lowest. 11D8 also binds to human albumin and so 11D8 is less suitable for immunopurification of huBChE  from plasma compared to e.g. B2 18-5.

Antibody First Published

Peng et al. Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads:KD values, binding   pairs, and amino acid sequences. Chem Biol Interact. 2015 Oct 5;240：336-45 PMID：26343001

Note on publication

Describes the characterization and   comparison of 5 monoclonal antibodies for their ability to purify huBChE on   Dynabeads.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.