Key features and details | |
Cat. No. | MABL-564 |
Name | Anti-c-erbB2 mAbs |
Clone No. | AFD- 520C9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, IP, therapeutic, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds an approximately 200 kD protein found in cancerous breast tissue which has been identified as c-erbB-2. |
Alternative Name | Breast cancer cells; CD340; Receptor tyrosine-protein kinase erbB-2; Neu; HER2; MLN19; Metastatic lymph node gene 19 protein; p185erbB2 |
UniProt | P04626 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with breast cancer cell lines. |
Application Notes | The antibody stained breast tumors and breast cancer lines (Ring et al., 1989; PMID： 2470501). The antibody was employed in immunocytochemical assay to identify breast tumor cells (Franklin et al., 1996; PMID： 8932871). Fab fragment of the antibody was generated and tested for in vivo efficacy in the MX-1human breast tumor cell, where it showed a significant decrease in tumor volume (US5169774A). Immunoprecipitation tests on the antibody indicated that it bound to a monomeric c.a.210,000 Da protein found in cancerous breast tissue with high association constant (US6054561A). The antibody was further evaluated for range breast cancer recognition by cell line immunofluorescence assays. The antibody showed positive results on SKBr3, BT483, CAMA1 (US5629197A). The antibody was employed in bi-specific and tri-specific antibody development to detect c-erbB2 expression and breast cancer treatment (Press et al. 2005; PMID：16166438). Furthermore, it was employed in developing tri-functional antibody ertumaxomab for breast cancer patients (Jager et al. 2009; PMID：19435924). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
