Key features and details | |
Cat. No. | MABL-562 |
Name | Anti-Butyrylcholinesterase mAbs |
Clone No. | AFD- C191 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody selectively captures completely denatured human butyrylcholinesterase. The antibody does not bind native butyrylcholinesterase. The antibody does not recognise mouse butyrylcholinesterase. |
Alternative Name | Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase; BChE |
UniProt | P06276 |
Immunogen | The original antibody was generated by injecting mice with denatured, recombinant human butyrylcholinesterase expressed in Chinese hamster ovary cells. The recombinant butyrylcholinesterase had been denatured and reduced by boiling in 1% sodium dodecyl sulfate containing 5% mercaptoethanol before it was injected into mice. |
Application Notes | The authors developed an antibody that can be used for immunopurifying heat- inactivated, denatured human butyrylcholinesterase. The specificity of the antibody (IgG1 format) for denatured butyrylcholinesterase was confirmed by ELISA analysis.The immobilized antibody was able to selectively extracts denatured butyrylcholinesterase from plasma. The selective removal of completely denatured butyrylcholinesterase by the immobilized antibody was visualized on Western blots. Mass spectrometry confirmed the finding from Western blots that the antibody immunoputifies butyrylcholinesterase from heat-inactivated plasma (Peng et al., 2016; PMID: 28058292). |
Antibody First Published | Peng et al. Monoclonal Antibodies That Recognize Various Folding States of Pure Human Butyrylcholinesterase Can Immunopurify Butyrylcholinesterase from Human Plasma Stored at Elevated Temperatures ACS Omega. 2016 Dec 31;1(6):1182-1191. PMID:28058292 |
Note on publication | The paper describes the generation and characterization of the antibody. The antibody was used to immunopurify denatured butyrylcholinesterase. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
