Anti-APP mAbs

Cat. No.

MABL-431

Name

Anti-APP mAbs

Clone No.

AFD- W20

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, in vitro, in vivo, SPR, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human amyloid   beta oligomers.

Alternative Name

 ABPP; APPI; Amyloid precursor protein; Alzheimer disease amyloid protein; Amyloid-beta precursor protein ; Alzheimer disease amyloid A4 protein homolog

UniProt

P05067

Immunogen

The original antibody was raised by panning  a human phage display library against amyloid beta.

Application Notes

The antibody was used for a nerve cell   toxicity assay. It significantly reduces the toxic effect of amyloid beta on   nerve cells. This antibody was used for a amyloid beta aggregation test where   it showed the inhibition of fibrosis aggregation of amyloid beta. This   antibody was used for an ELISA on amyloid beta oligomers. This antibody was   used for surface plasmon resonance to determine its binding to amyloid beta.   This antibody was used for a western blot on amyloid beta oligomers   (CN101463082A). This antibody was used in vivo on A53T α-synuclein mice. When   treated with this antibody it showed significant improvement in rotarod   latency (Zha et al., 2016; PMID:27824125). This antibody conjugated to   superparamagnetic iron oxide nanoparticles was used in vivo in a mouse model   for Alzheimer’s disease.

Antibody First Published

Zhao et al. Pan-amyloid oligomer specific   scFv antibody attenuates memory deficits and brain amyloid burden in mice   with Alzheimer's disease Curr Alzheimer Res. 2014 Jan;11(1)-69:78.   PMID:24156260

Note on publication

The original paper showed that W20 blocked the   binding of Aβ oligomers to SH-SY5Y cells, did not bind to heat shock protein,  rescued cognitive impairments in APP/PS1 transgenic mice, and interfered with   Aβ levels and deposits in mouse brain.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.