Anti-Apical membrane antigen 1 mAbs

Cat. No.

MABL-422

Name

Anti-Apical membrane antigen 1 mAbs

Clone No.

AFD- F8.12.19

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, WB, ELISA, IF

Species Reactivity

Plasmodium spp

Basic Information

Specificity

This antibody recognizes a discontinuous conformational   epitope located on domain III of the ectoplasmic region of the apical   membrane antigen 1 (AMA1) of Plasmodium vivax and also cross reacts with AMA1   from Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium berghei and   Plasmodium falciparum. AMA1 is involved in parasite invasion of erythrocytes.

Alternative Name

AMA1;AMA-1; Apical merozoite antigen 1;

UniProt

Q9BIM8; Q9TY14

Immunogen

 The original antibody was generated by   immunizing BALB/c mice with recombinant ectoplasmic region of AMA1 from   Plasmodium vivax.

Application Notes

This antibody can recognize AMA1 of   Plasmodium vivax, Plasmodium knowlesi, Plasmodium cynomolgi, Plasmodium   berghei and Plasmodium falciparum in an immunofluorescence assay on mature   schizonts. The binding region is a discontinuous epitope located on domain   III of the ectoplasmic region, the major component being a loop containing a   cystine knot. The binding of F8.12.19 to recombinant AMA1 from both P. vivax   and P. falciparum was measured by surface plasmon resonance. This antibody   shows no function-blocking effect was detected in ex vivo parasite growth   inhibition assays. (PMID: 17229439). This antibody a dilution of 1 16,000 was   used for the detection of recombinant PvAMA-1 and recombinant PvAMA-DII-III   using dot-blot and ELISA assays (PMID: 28855657). This antibody was also used   for the identification of PfAMA1 using western blot (PMID: 25236489).

Antibody First Published

Igonet et al. Cross-reactivity studies of an   anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody  binding and structural characterization. J   Mol Biol. 2007 Mar 9;366(5):1523-37. PMID:17229439

Note on publication

This paper analysis the cross reactivity of   an anti-AMA1 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.